Induced Pluripotent Stem Cell (iPSC) Core

Overview of Services

The induced Pluripotent Stem Cells (iPSC) Core was created in 2011 to accelerate stem cell research in the NHLBI by providing investigators consultation, technical services and training in human pluripotent stem cell technology. The major services that the Core currently provides include (1) generation of human iPSCs from fibroblast cells, CD34+ hematopoietic stem/progenitor cells, and peripheral blood mononuclear cells (PBMCs) using non-integration methods; (2) CRISPR/Cas9 mediated human iPSC gene knockout and AAVS1 safe harbor transgene knockin; (3) human iPSC-cardiomyocyte (CM) differentiation. The Core also provides several wild-type and fluorescent-protein/luciferase reporter control iPSC lines, as well as validated iPSC culture reagents. In addition to providing services and consultations to researchers from various disciplines, the Core staff is developing new human iPSC gene-editing technologies, establishing lineage-specific differentiation protocols and collaborates with investigators in specific projects. Research conducted by the Core seeks to better understand the mechanisms underlying pluripotent stem cell self-renewal and differentiation to optimize culture conditions and operating procedures.

iPSC repositories:

https://www.wicell.org/home/stem-cells/cell-line-collections/collections.cmsx

https://www.fujifilmcdi.com/cirm-ipsc-products/

https://www.coriell.org/Search?grid=0&q=*~*&csId=&f_2=iPSC

Leadership

Jizhong Zou, Ph.D. | Core Director

Location and hours of operation

Getting Started

1. To obtain a login and password, click on sign-up

2. Once you receive your login credentials, login above

3. After logging in, click on the "List All Cores" link on the left to locate iPSC Core at NHLBI